CMA Substrates

S.No. Protein Species UniProt ID Length Canonical KFERQ-like motif Potential Motifs No. of Canonical KFERQ-like motif Same-protein structural representation (%) Homolog-derived structural representation (%) References
1 Hemoglobin subunit beta Human P68871 147 40QRFFE - Single 100 100 1,2
2 Alpha-synuclein Human P37840 140 95VKKDQ - Single 100 100 3,4,5,6,7,8,9,10,11,12
3 Annexin A1 Human P04083 346 6EFLKQ - Single 97.97 100 13,14
4 Pyruvate kinase PKM Rabbit P11974 531 227QDLKF 132VELKK Single 100 100 1,12,13,15,16,17,18
5 Perilipin-3 Mouse Q9DBG5 437 100LDRLQ - Single 56.52 0 4,12,19
5 Perilipin-3 Human O60664 434 244ERLRQ, 288QKLVE - Multiple 0 56.91 20
6 Stimulator of interferon genes protein Mouse Q3TBT3 378 326QEVLR - Single 55.82 99.73 6,12,21
7 Phospholipid hydroperoxide glutathione peroxidase GPX4 Mice O70325 197 187QVIEK - Single 85.78 86.29 22,23
8 Cystic fibrosis transmembrane conductance regulator Human P13569 1480 30QRLEL 170RVLDK, 810RRLSQ, 1186QLSKV, 1386RTLKQ, 1435SLFRQ Single 100 100 20
9 Ribonuclease pancreatic Bovin P61823 150 33KFERQ - Single 100 86.66 1,12,24,25
9 Ribonuclease pancreatic Human P07998 156 - - - 82.69 85.25 1,12,24,25
10 TAR DNA-binding protein 43 Human Q13148 414 134QVKKD 136KKDLK Single 100 56.03 6,12,26
11 Parkinson disease protein 7 Human Q99497 189 91ILKEQ 89KEILK Single 100 100 12,27
12 Annexin A2 Human P07355 339 226QKVFD 227KVFDR, 245RKEVK, 306EFKRK Single 100 100 1,13,14
13 Annexin A4 Human P09525 319 93QELRR 21QTLRK, 122RRISQ, 213KRISQ Single 100 100 1,13,14
14 Major prion protein Human P04156 253 208RVVEQ 182ITIKQ Single 82.6 100 6,12,28
15 Fructose-bisphosphate aldolase A Mouse P05064 364 12QKKEL 57RFYRQ, 96QVIKS, 107IKVDK Single 0 100 12,15
16 Astrocytic phosphoprotein PEA-15 Human Q15121 130 110DIIRQ 109 KDIIR Single 100 100 6,12,18,29
17 Rho-related GTP-binding protein RhoE Human P61587 244 69QRIEL 233 LRKDK Single 87.7 87.7 12,18,30,31
18 Annexin A6 Human P08133 673 564QEFIK 81 KFERL, 258ERLFK, 350RVELK Single 99.85 100 13,14
19 Serine/threonine-protein kinase Chk1 Human O14757 476 336DKLVQ 180KRREF Single 85.5 97.89 12,32
20 Lysosomal acid glucosylceramidase Human P04062 536 295QRDFI 389QSVRL Single 100 80.03 6,33
21 G1/S-specific cyclin-D1 Human P24385 295 49QKEVL 96KSRLQ Single 100 100 6,34
22 Aspartate aminotransferase (cytoplasmic) Human P17174 413 55KKVEQ 97KEKRV, 409VTKIQ Single 100 100 1
23 Interleukin-6 receptor subunit beta Human P40189 918 884QVERF 49VLKEK,643KRDLI,644RDLIK,645DLIKK,712DLFKK,714FKKEK,715KKEKI, 775QVFSR Single 73.96 100 35,36
24 U2 small nuclear ribonucleoprotein A Mice P57784 225 152LKERQ 151KLKER, 217VERLK Single 0 100 37,38
25 Homeobox protein Hox-B13 Human Q92826 284 227QLREL 268RVKEK, 269VKEKK, 270KEKKV Single 26.76 34.15 38,39
26 calreticulin Mice P14211 416 365QRLKE 94QFTVK, 206KKIKD Single 53.12 85.33 38,40
27 Amyloid-beta precursor protein Human P05067 770 763KFFEQ 347QSLLK Single 100 74.54 6,12,41
28 Pyruvate kinase PKM Human P14618 531 227QDLKF 132 VELKK Single 100 100 1,12,13,15,16,17,18
29 Intraflagellar transport protein 88 homolog Human Q13099 824 136EKIKQ 174KERVL, 242IYLKQ, 329QKLIT, 439KVLEK, 440VLEKK, 587QVLSK, 714KRLEK, 725QRIKS,804KKRID Single 0 87.99 20
30 E3 ubiquitin-protein ligase Mdm2 Human Q00987 491 135QKDLV, 136KDLVQ - Multiple 100 38.09 6,12,42
31 Aspartate aminotransferase, cytoplasmic Pig P00503 413 55RKVEQ, 97QEKRV 283RVLSQ, 409VTKIQ Multiple 100 100 12,13,17
32 Long-chain specific acyl-CoA dehydrogenase, mitochondrial Mouse P51174 430 153QIEKF, 290QERLL - Multiple 0 96.97 12,15
33 Transcription factor EB Mice Q9R210 475 89QKVRE, 217QKREL - Multiple 0 100 43,44
34 Fructose-bisphosphate aldolase B Human P05062 364 12QKKEL, 58QFREI, 107IKLDQ 97ILKEK Multiple 100 100 1,13,45
35 Paired box protein Pax-2 Human Q02962 417 35DVVRQ, 39QRIVE 57QLRVS, 94KVVDK, 140RTKVQ Multiple 0 35.01 1,6,13,46,47
36 Hexokinase-2 Human P52789 917 142 QIKDK, 291QLFEK, 395QRIKE, 739QRFEK 143IKDKK,144KDKKL, 785KFLSQ Multiple 98.25 100 6,12,48
37 Epidermal growth factor receptor kinase substrate 8 Human Q12929 822 226QVDVR, 470QEIKR 74VLDRK, 284ITKLQ, 680VDRRK Multiple 17.88 35.76 13,18,49
38 Heat shock cognate 71 kDa protein Human P11142 646 532QRDKV, 568QKILD 244EFKRK, 569KILDK Multiple 75.85 97.98 1,50
39 Myc proto-oncogene protein Human P01106 439 282VEKRQ, 376VLERQ 66KKFEL, 426QKLIS, 433DLLRK Multiple 47.38 18.9 12,51
40 NF-kappa-B inhibitor alpha Human P25963 317 46VKELQ, 50QEIRL, 92EVIRQ 21KKERL, 22KERLL, 313QRLTL Multiple 84.22 77.28 1,13,44
41 Ubiquilin-1 Human Q9UMX0 589 84ILKDQ, 544EVRFQ 83KILKD, 105VIKTQ Multiple 21.22 27.5 12,52
42 Hepatocyte growth factor-like protein Human P26927 711 229KFLDQ, 511LVKEQ 470KRVDR Multiple 34.73 97.6 6,53
43 Nuclear receptor corepressor 1 Human O75376 2440 348EIRKQ, 450DKFIQ, 1317QIKRE, 2210QEIFR 233RSIVQ, 320KKVDR, 321KVDRI, 445KEIFK, 447IFKDK, 449KDKFI, 1428KVVER, 1875KRSVQ, 1936IITRQ, 2211EIFRK, 2265DIIRK Multiple 0.05 14.91 4,6,18,54
44 Protein CIP2A Human Q8TCG1 905 601IEKLQ, 609VVKDQ, 779QLIEK, 786QRKEV, 787RKEVQ, 793QLVDR, 841 QIRKE 39QKLTR, 501YKILQ, 806QKTKV, 807KTKVQ, 815IKTLQ, 830DILRK Multiple 61.87 0 6,12,51
45 ATPase MORC2 Human Q9Y6X9 1032 290QEVKK, 566EKIRQ 263QTKRL, 350KRIKE, 665SRLLQ, 825KVKFD, 929ISKKQ, 995QKLRT Multiple 100 52.62 31,55
46 E3 ubiquitin-protein ligase Itchy Mouse Q8C863 864 532QDLRR, 775QFVKE, 846QLKEK 377QRFIY, 486VRDFK, 494QYFRF, 848KEKLL Multiple 83.56 90.5 4,12,56
47 Proto-oncogene vav Human P15498 845 199LREIQ, 341QELVK, 697QRVKD 274KERFL, 489RELKK, 490ELKKK, 576KKDKL Multiple 93.25 95.14 18,57
48 Leucine-rich repeat serine/threonine-protein kinase 2 Human Q5S007 2527 412KEVFQ, 769QDVRK, 1379QIRDK, 1512KIRDQ, 1555QLVRE, 1653LEKFQ, 1956DRLLQ, 2333QKLIE 52SKLFQ, 843VIRYQ, 1128LKELK, 1129KELKI, 1178LKLSQ, 1256KLKEI, 1316KDIIR, 1385KRDLV, 1547VIDRK, 1648QYFKL, 1651KLLEK, 2108EKLIK, 2511IEVRK Multiple 100 61.1 4,6,12,13,58
49 Huntingtin Human P42858 3142 904QERVL, 1567QKEVV, 1759QLKVE 14LKSFQ, 96KKDRV, 172KEIKK, 382QLFRT, 435LSRKQ, 1314QLLKT, 1605RLSRQ, 1693LSRIQ, 1755IVTKQ, 1971KKTLQ, 2103LVKSQ, 2247KDIVK, 2433KEVFK, 2890QLSRL, 2963DRIRK, 3017YKVFQ Multiple 100 99.45 4,12,59,60,61
50 Glycerol-3-phosphate dehydrogenase, mitochondrial Mouse Q64521 727 208QKDKL, 585ELRKQ, 649QRVLE 119VRYLQ, 209KDKLV Multiple 0 72.35 12,15
51 Vacuolar protein sorting-associated protein 35 Human Q96QK1 796 12QEKLL, 214QELRI, 748QKIRE 127KDILK, 225VRLSQ, 558QKIFS Multiple 100 100 6,62
52 Microtubule-associated protein tau Human P10636 758 653QVEVK, 664KDRVQ 557KSRLQ, 597KKLDL, 624QIVYK, 660KLDFK Multiple 100 56.87 4,6,12,63,64,65
53 Ryanodine receptor 2 Rat B0LPN4 4955 1003 DRIRQ, 2651QELFK, 2882QDIFK, 2950QEIKF, 3815EKVLQ, 4410QKVKE 12QFLRT, 249QRRTV, 638QTRLV, 822KEKLK, 824KLKVE, 952EKVKK, 1276LKVTQ, 1375SRLKQ, 1868KLEVK, 1932QRFRY, 2117QIRSL, 2145KVFYQ, 2197RISRQ, 2797RRISQ, 2920QLIRY, 3305QLLKT, 3316EKLKK, 3570LLSKQ, 3710QKLLY, 3711KLLYQ, 3769LKEKK, 4010KLKDL, 4131KRIER, 4251SLKKQ, 4378QYKLI, 4588FKREK, 4634DKFVK Multiple 0.002 3.51 4,6,66
54 Histone acetyltransferase p300 Human Q09472 2414 1485DIFKQ, 1663QDRFV, 2178QFRDI 637 IYKIQ, 649RTRLQ, 1103KRKLD, 1230QFSKR, 1390QRRVY, 1484KDIFK, 1732RLSIQ Multiple 41.01 42.62 6,15,67
55 Bcl-2-binding component 3, isoforms 1/2 Human Q9BXH1 193 - Advanced motif (169RVLYN) - 15.02 13.47 6,68
56 Endoribonuclease Dicer Human Q9UPY3 1922 224QKLEK, 326VRELQ, 497EFRKQ, 1702QRLEF 225KLEKI, 227EKILK, 297LISKQ, 330QKYIK, 382EILRK, 503EVLRK, 829IELKK, 858LRLEK Multiple 100 100 69
57 NACHT, LRR and PYD domains-containing protein 3 Human Q96P20 1036 355LEKLQ, 603QIRLE, 798QKLVE, 991EVLKQ 20VDLKK, 145RSRFQ, 969QSLRK Multiple 100 9.85 70
58 Aspartate aminotransferase( mitochondrial) Human P00505 430 335DLRKQ, 342QEVKV, 390QVERL 392ERLIK Multiple 100 100 1
59 Alpha-2-macroglobulin Human P01023 1474 112QEFKK, 228KFEVQ 142QTVKF, 303QVKTK, 306TKVFQ, 823RVSVQ, 1162KRKEV, 1164KEVLK, 1439VRDLK Multiple 100 99.32 1
60 S-glutathionylates nitric oxide synthase(eNOS) Human P29474 1203 677ERLLQ, 1089QDILR, 1148VLRDQ 247QLVRY, 733QRYRL, 1172RIRTQ Multiple 39.81 95.42 71
61 Transcriptional coactivator YAP1 Human P46937 504 323QELLR, 324ELLRQ 186QRYFL, 315KERLR Multiple 55.95 42.46 35,36
62 glucose-6 phosphate dehydrogenase (G6PD) Human P11413 515 79DIRKQ, 368EVRLQ 95KLKLE, 195QIYRI Multiple 100 97.86 15,72
62 glucose-6 phosphate dehydrogenase (G6PD) Rat P05370 515 79DIRKQ, 368EVRLQ, 372QFRDV 195QIYRI Multiple 0 100 15,72
63 Serine/threonine-protein kinase TBK1 Human Q9UHD2 729 549VEKLQ, 565QFKKD, 584KFDKQ 57EVLKK, 338LVYKQ, 342QTKII, 566FKKDK, 691KKLKE Multiple 100 90.12 72,73
64 Pericentrin Human O95613 3336 305LLREQ, 329KEKLQ, 392EKIFQ, 1534QKLRE, 1746IEKLQ, 1947LDRRQ, 2494IIREQ, 2719QKELR, 2792QKLKE, 2808LEKVQ, 2853QKREL, 2881RLKEQ 341QIVKT, 435EFKFK, 518QLRIY, 525KKLRD, 716LEKVK, 788KFELR, 1016LEKLK, 1072TLRLQ, 1367SLRRQ, 1535KLREK, 1599KEVLK, 1600EVLKK, 1928RLSRQ, 2052KEKVL, 2228EVLRK, 2493KIIRE, 2603QTVVR, 2606VRDLK, 2720KELRI, 2833LRREK, 2854KRELR, 2914KEKLR, 2916KLREL, 2980QRLLS, 3075KLEKL, 3077EKLLK, 3152QKKYL Multiple 0 0 20
65 Vimentin Human P08670 466 104KVELQ, 122RFLEQ, 156ELRRQ, 186REKLQ, 270RDVRQ 184RLREK Multiple 100 68.45 20
66 Serine/threonine-protein kinase ULK1 Mice O70405 1051 227QDLRL, 962FLDKQ, 966QRLLD 62KEIKI, 65KILKE, 67LKELK, 955SLRLQ Multiple 0 49.48 38,74
67 E3 ubiquitin-protein ligase TRIM21 Mice Q62191 470 202QRLEK, 249QEVRI 148EKLRK, 178QKSRI Multiple 38.08 100 38,75
68 Signal transducer and activator of transcription 3 Human P40763 770 149QDVRK, 322VVERQ 69SRFLQ, 113SRLLQ, 570DLVKK Multiple 78.96 95.32 76
69 Cellular tumor antigen p53 Human P04637 393 - Advanced motifs (200NLRVE, 341FRELN, 369LKSKK) - 100 55.72 4,12,77
70 Epidermal growth factor receptor Human P00533 1210 - 79LKTIQ, 208QKLTK, 486QKTKI, 960KFREL, 976QRYLV - 100 85.37 4,78
71 Sorting nexin-10 Human Q9Y5X0 201 - 100DFLRK - 100 80.59 31,79
72 Citrate synthase, mitochondrial Mouse Q9CZU6 464 - 49KTFKQ, 379QLYKI - 1.5 100 12,15
73 Myocyte-specific enhancer factor 2A Human Q02078 507 - 7QITRI - 21.1 18.73 4,6,80
74 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Human P09936 223 - 112SVLKQ - 100 97.75 4,6,81,82
75 Peroxisomal multifunctional enzyme type 2 Human P51659 736 - 80EKVVK, 359KDLKF, 641EVVKK - 97.96 82.33 6,83
76 Translationally-controlled tumor protein Human P13693 172 - 19KIREI - 100 100 6,12,84
77 High mobility group protein B1 Human P09429 215 - 87KKFKD, 150KLKEK - 100 79.53 6,85
78 Induced myeloid leukemia cell differentiation protein Mcl-1 Human Q07820 350 - 234KLDIK - 58.28 51.42 6,86
79 Sterol 26-hydroxylase, mitochondrial Mouse Q9DBG1 533 - 285KKLID, 494SRLVQ - 0 98.5 12,15
80 Hypoxia-inducible factor 1-alpha Human Q16665 826 - 164QRSFF, 373QLFTK, 387FDKLK, 388DKLKK, 389KLKKE, 573QLRSF, 768QKTII - 60.9 42.97 12,13,31,87,88
81 Malate dehydrogenase, cytoplasmic Mouse P14152 334 - 99KDLLK, 108IFKSQ - 100 100 12,15
82 Centrin-1 Human Q12798 172 - 51KELKV - 0 100 20
83 ADP-ribosylation factor-like protein 13B Human Q3SXY8 428 - 173KKIDK, 221ERVRK - 0 53.27 20
84 Protein deacetylase HDAC6 Human Q9UBN7 1215 - 49EVKKK, 872KKVLE - 38.27 76.46 20
85 DNA damage-inducible transcript 4 protein Mice Q9D3F7 229 - 126KELLR - 0 100 38,89,90
85 DNA damage-inducible transcript 4 protein Human Q9NX09 232 - 129KELLR - 100 0 38,89,90
86 Protein c-Fos Rat P12841 380 - 186LLKEK - 0 16.84 13,91
87 Kelch-like ECH-associated protein 1 Human Q14145 624 - 70LRLSQ, 259QRRFY, 261RFYVQ - 100 94.07 36,92
88 Myocyte-specific enhancer factor 2D Human Q14814 521 - Advanced motif (21FTKRK, 87TLRKK) - 18.04 18.23 1,2,93,94
89 Perilipin-2 Mouse P43883 425 - 414SLKVQ - 0 56 4,12,19
89 Perilipin-2 Human Q99541 437 - Advanced motifs (14TRVVN, 236LSRVK, 355SVFRN) - 0 54.46 20
90 Protein AF1q Human Q13015 90 - 84SFELD (No such motif found using KFERQ finder) - 0 0 4,6,18,95
91 Phosphatidylethanolamine-binding protein 1 Human P30086 187 - Advanced motifs (153SFRKK) - 100 100 4,96
92 Glyceraldehyde-3-phosphate dehydrogenase Rat P04797 333 - Advanced motifs (140NSLKI, 143KIVSN, 320NRVVD) - 0 100 13,44,46,97,98
93 Calcipressin-1 Human P53805 252 - Advanced motifs (103FKSFK, 104KSFKR) - 14.68 36.9 4,6,13,56,99
94 Glutathione transferase Species not clear - - - NKKFE (GST can be a substrate of CMA under specific conditions, such as during ischemia-reperfusion injury where the enzyme, specifically GSTP,S-glutathionylates nitric oxide synthase(eNOS), leading to its degradation via CMA) - - - 1,71
95 Galectin-3 Human P17931 250 No such motif found using KFERQ finder No such motif found using KFERQ finder - 62 99.6 4,100
96 Polyubiquitin-C Human P0CG48 685 No such motif found using KFERQ finder No such motif found using KFERQ finder - 100 100 20
97 Polyubiquitin-B Human P0CG47 229 No such motif found using KFERQ finder No such motif found using KFERQ finder - 100 100 20
98 Ubiquitin-ribosomal protein eL40 fusion protein Human P62987 128 No such motif found using KFERQ finder No such motif found using KFERQ finder - 100 100 20
99 Ubiquitin-ribosomal protein eS31 fusion protein Human P62979 156 - Advanced motifs (113KISRL) - 100 100 20