CMA Substrates

S.No.	Protein	Species	UniProt ID	Length	Canonical KFERQ-like motif	Potential Motifs	No. of Canonical KFERQ-like motif	% of residues experimentally resolved	References
1	Hemoglobin subunit beta	Human	P68871	147	40QRFFE	-	Single	100	1,2
2	Alpha-synuclein	Human	P37840	140	95VKKDQ	-	Single	100	3,4,5,6,7,8,9,10,11,12
3	Annexin A1	Human	P04083	346	6EFLKQ	-	Single	100	13,14
4	Pyruvate kinase PKM	Rabbit	P11974	531	227QDLKF	-	Single	100	1,12,13,15,16,17,18
5A	Perilipin-3	Mouse	Q9DBG5	437	100LDRLQ	-	Single	56.52	4,12,19
5B	Perilipin-3	Human	O60664	434	244ERLRQ, 288QKLVE	-	Multiple	56.91	20
6	Stimulator of interferon genes protein	Mouse	Q3TBT3	378	326QEVLR	-	Single	99.74	6,12,21
7	Phospholipid hydroperoxide glutathione peroxidase GPX4	Mice	O70325	197	187QVIEK	-	Single	83.7	22,23
8	Cystic fibrosis transmembrane conductance regulator	Human	P13569	1480	30QRLEL	-	Single	100	20
9A	Ribonuclease pancreatic	Bovin	P61823	150	33KFERQ	-	Single	100	12,24,25
9B	Ribonuclease pancreatic	Human	P07998	156	-	5KSLVR	Single	85.9	1,24,25
10	TAR DNA-binding protein 43	Human	Q13148	414	134QVKKD	136KKDLK	Single	100	6,12,26
11	Parkinson disease protein 7	Human	Q99497	189	91ILKEQ	89KEILK	Single	100	12,27
12	Annexin A2	Human	P07355	339	226QKVFD	227KVFDR, 245RKEVK, 306EFKRK	Single	100	1,13,14
13	Annexin A4	Human	P09525	319	93QELRR	21QTLRK, 122RRISQ, 213KRISQ	Single	100	1,13,14
14	Major prion protein	Human	P04156	253	208RVVEQ	182ITIKQ	Single	100	6,12,28
15	Fructose-bisphosphate aldolase A	Mouse	P05064	364	12QKKEL	57RFYRQ, 96QVIKS, 107IKVDK	Single	100	12,15
16	Astrocytic phosphoprotein PEA-15	Human	Q15121	130	110DIIRQ	109KDIIR	Single	100	6,12,18,29
17	Rho-related GTP-binding protein RhoE	Human	P61587	244	69QRIEL	233LRKDK	Single	94.26	12,18,30,31
18	Annexin A6	Human	P08133	673	564QEFIK	81KFERL, 258ERLFK, 350RVELK	Single	100	13,14
19	Serine/threonine-protein kinase Chk1	Human	O14757	476	336DKLVQ	180KRREF	Single	100	12,32
20	Lysosomal acid glucosylceramidase	Human	P04062	536	295QRDFI	389QSVRL	Single	100	6,33
21	G1/S-specific cyclin-D1	Human	P24385	295	49QKEVL	96KSRLQ	Single	100	6,34
22	Aspartate aminotransferase (cytoplasmic)	Human	P17174	413	55KKVEQ	97KEKRV, 409VTKIQ	Single	100	1
23	Interleukin-6 receptor subunit beta	Human	P40189	918	884QVERF	49VLKEK, 643KRDLI, 644RDLIK, 645DLIKK, 712DLFKK, 714FKKEK, 715KKEKI, 775QVFSR	Single	100	35,36
24	U2 small nuclear ribonucleoprotein A	Mice	P57784	225	152LKERQ	151KLKER, 217VERLK	Single	100	37,38
25	Homeobox protein Hox-B13	Human	Q92826	284	227QLREL	268RVKEK, 269VKEKK, 270KEKKV	Single	34.86	38,39
26	calreticulin	Mice	P14211	416	365QRLKE	94QFTVK, 206KKIKD	Single	85.34	38,40
27	Amyloid-beta precursor protein	Human	P05067	770	763KFFEQ	347QSLLK	Single	100	6,12,41
28	Pyruvate kinase PKM	Human	P14618	531	227QDLKF	132VELKK	Single	100	1,12,13,15,16,17,18
29	Intraflagellar transport protein 88 homolog	Human	Q13099	824	145EKIKQ	183KERVL, 251IYLKQ, 448KVLEK, 449VLEKK, 596QVLSK, 723KRLEK, 734QRIKS, 813KKRID	Single	87.99	20
30	E3 ubiquitin-protein ligase Mdm2	Human	Q00987	491	135QKDLV, 136KDLVQ	-	Multiple	100	6,12,42
31	Aspartate aminotransferase, cytoplasmic	Pig	P00503	413	55RKVEQ, 97QEKRV	-	Multiple	100	12,13,17
32	Long-chain specific acyl-CoA dehydrogenase, mitochondrial	Mouse	P51174	430	153QIEKF, 290QERLL	-	Multiple	96.98	12,15
33	Transcription factor EB	Mice	Q9R210	475	89QKVRE, 217QKREL	-	Multiple	100	43,44
34	Fructose-bisphosphate aldolase B	Human	P05062	364	12QKKEL, 58QFREI, 107IKLDQ	97ILKEK	Multiple	100	1,13,45
35	Paired box protein Pax-2	Human	Q02962	417	35DVVRQ, 39QRIVE	57QLRVS, 94KVVDK, 140RTKVQ	Multiple	35.01	1,6,13,46,47
36	Hexokinase-2	Human	P52789	917	142QIKDK, 291QLFEK, 395QRIKE, 739QRFEK	143IKDKK, 144KDKKL, 785KFLSQ	Multiple	100	6,12,48
37	Epidermal growth factor receptor kinase substrate 8	Human	Q12929	822	226QVDVR, 470QEIKR	74VLDRK, 284ITKLQ, 680VDRRK	Multiple	36.67	13,18,49
38	Heat shock cognate 71 kDa protein	Human	P11142	646	532QRDKV, 568QKILD	244EFKRK, 569KILDK	Multiple	100	1,50
39	Myc proto-oncogene protein	Human	P01106	439	267VEKRQ, 361VLERQ	51KKFEL, 411QKLIS, 418DLLRK	Multiple	45.81	12,51
40	NF-kappa-B inhibitor alpha	Human	P25963	317	46VKELQ, 50QEIRL, 92EVIRQ	21KKERL, 22KERLL, 313QRLTL	Multiple	88.96	1,13,44
41	Ubiquilin-1	Human	Q9UMX0	589	84ILKDQ, 105VIKTQ	83KILKD, 105VIKTQ	Multiple	27.5	12,52
42	Hepatocyte growth factor-like protein	Human	P26927	711	229KFLDQ, 511LVKEQ	470KRVDR	Multiple	98.03	6,53
43	Nuclear receptor corepressor 1	Human	O75376	2440	348EIRKQ, 450DKFIQ, 1317QIKRE, 2210QEIFR	233RSIVQ, 320KKVDR, 321KVDRI, 445KEIFK, 447IFKDK, 449KDKFI, 1428KVVER, 1875KRSVQ, 1936IITRQ, 2211EIFRK, 2265DIIRK	Multiple	17.38	4,6,18,54
44	Protein CIP2A	Human	Q8TCG1	905	601IEKLQ, 609VVKDQ, 779QLIEK, 786QRKEV, 787RKEVQ, 793QLVDR, 841QIRKE	39QKLTR, 501YKILQ, 806QKTKV, 807KTKVQ, 815IKTLQ, 830DILRK	Multiple	61.88	6,12,51
45	ATPase MORC2	Human	Q9Y6X9	1032	290QEVKK, 566EKIRQ	263QTKRL, 350KRIKE, 665SRLLQ, 825KVKFD, 929ISKKQ, 995QKLRT	Multiple	100	31,55
46	E3 ubiquitin-protein ligase Itchy	Mouse	Q8C863	864	532QDLRR, 775QFVKE, 846QLKEK	377QRFIY, 486VRDFK, 494QYFRF, 848KEKLL	Multiple	100	4,12,56
47	Proto-oncogene vav	Human	P15498	845	199LREIQ, 341QELVK, 697QRVKD	274KERFL, 489RELKK, 490ELKKK, 576KKDKL	Multiple	99.88	18,57
48	Leucine-rich repeat serine/threonine-protein kinase 2	Human	Q5S007	2527	412KEVFQ, 769QDVRK, 1379QIRDK, 1512KIRDQ, 1555QLVRE, 1653LEKFQ, 1956DRLLQ, 2333QKLIE	57SKLFQ, 843VIRYQ, 1128LKELK, 1129KELKI, 1178LKLSQ, 1256KLKEI, 1316KDIIR, 1385KRDLV, 1547VIDRK, 1648QYFKL, 1651KLLEK, 2108EKLIK, 2511IEVRK	Multiple	100	4,6,12,13,58
49	Huntingtin	Human	P42858	3142	904QERVL, 1567QKEVV, 1759QLKVE	14LKSFQ, 96KKDRV, 172KEIKK, 382QLFRT, 435LSRKQ, 1314QLLKT, 1605RLSRQ, 1693LSRIQ, 1755IVTKQ, 1971KKTLQ, 2103LVKSQ, 2247KDIVK, 2433KEVFK, 2890QLSRL, 2963DRIRK, 3017YKVFQ	Multiple	100	4,12,59,60,61
50	Glycerol-3-phosphate dehydrogenase, mitochondrial	Mouse	Q64521	727	208QKDKL, 585ELRKQ, 649QRVLE	119VRYLQ, 209KDKLV	Multiple	72.35	12,15
51	Vacuolar protein sorting-associated protein 35	Human	Q96QK1	796	12QEKLL, 214QELRI, 748QKIRE	127KDILK, 225VRLSQ, 558QKIFS	Multiple	100	6,62
52	Microtubule-associated protein tau	Human	P10636	758	336QVEVK, 347KDRVQ	557KSRLQ, 597KKLDL, 624QIVYK, 660KLDFK, 664KDRVQ	Multiple	100	4,6,12,63,64,65
53	Ryanodine receptor 2	Rat	B0LPN4	4953	1003DRIRQ, 2649QELFK, 2880QDIFK, 2948QEIKF, 3813EKVLQ, 4408QKVKE	12QFLRT, 249QRRTV, 638QTRLV, 822KEKLK, 824KLKVE, 952EKVKK, 1276LKVTQ, 1375SRLKQ, 1868KLEVK, 1932QRFRY, 2115QIRSL, 2143KVFYQ, 2195RISRQ, 2795RRISQ, 2918QLIRY, 3303QLLKT, 3314EKLKK, 3568LLSKQ, 3708QKLLY, 3709KLLYQ, 3767LKEKK, 4008KLKDL, 4129KRIER, 4249SLKKQ, 4376QYKLI, 4586FKREK, 4632DKFVK	Multiple	100	4,6,66
54	Histone acetyltransferase p300	Human	Q09472	2414	1485DIFKQ, 1663QDRFV, 2178QFRDI	637IYKIQ, 649RTRLQ, 1103KRKLD, 1230QFSKR, 1390QRRVY, 1484KDIFK, 1732RLSIQ	Multiple	42.67	6,15,67
55	Bcl-2-binding component 3, isoforms 1/2	Human	Q9BXH1	193	-	Advanced motif (169RVLYN)	-	15.03	6,68
56	Endoribonuclease Dicer	Human	Q9UPY3	1922	224QKLEK, 326VRELQ, 497EFRKQ, 1702QRLEF	225KLEKI, 227EKILK, 297LISKQ, 330QKYIK, 382EILRK, 503EVLRK, 829IELKK, 858LRLEK	Multiple	100	69
57	NACHT, LRR and PYD domains-containing protein 3	Human	Q96P20	1036	355LEKLQ, 603QIRLE, 798QKLVE, 991EVLKQ	20VDLKK, 145RSRFQ, 969QSLRK	M ultiple	100	70
58	Aspartate aminotransferase (mitochondrial)	Human	P00505	430	335DLRKQ, 342QEVKV, 390QVERL	392ERLIK	Multiple	100	1
59	α2-Macroglobulin	Human	P01023	1474	112QEFKK, 228KFEVQ	142QTVKF, 303QVKTK, 306TKVFQ, 823RVSVQ, 1162KRKEV, 1164KEVLK, 1439VRDLK	Multiple	100	1
60	S-glutathionylates nitric oxide synthase (eNOS)	Human	P29474	1203	677ERLLQ, 1089QDILR, 1148VLRDQ	247QLVRY, 733QRYRL, 1172RIRTQ	Multiple	96.01	71
61	Transcriptional coactivator YAP1	Human	P46937	504	323QELLR, 324ELLRQ	186QRYFL, 315KERLR	Multiple	65.67	35,36
62A	Glucose-6 phosphate dehydrogenase (G6PD)	Human	P11413	515	79DIRKQ, 368EVRLQ	95KLKLE, 195QIYRI	Multiple	100	15,72
62B	Glucose-6-phosphate 1-dehydrogenase	Rat	P05370	515	79DIRKQ, 368EVRLQ, 372QFRDV	195QIYRI	Multiple	100	15,72
63	Serine/threonine-protein kinase TBK1	Human	Q9UHD2	729	549VEKLQ, 565QFKKD, 584KFDKQ	57EVLKK, 338LVYKQ, 342QTKII, 566FKKDK, 691KKLKE	Multiple	100	72,73
64	Pericentrin	Human	O95613	3336	305LLREQ, 329KEKLQ, 392EKIFQ, 1534QKLRE, 1746IEKLQ, 1947LDRRQ, 2494IIREQ, 2719QKELR, 2792QKLKE, 2808LEKVQ, 2853QKREL, 2881RLKEQ	341QIVKT, 435EFKFK, 518QLRIY, 525KKLRD, 716LEKVK, 788KFELR, 1016LEKLK, 1072TLRLQ, 1367SLRRQ, 1535KLREK, 1599KEVLK, 1600EVLKK, 1928RLSRQ, 2052KEKVL, 2228EVLRK, 2493KIIRE, 2603QTVVR, 2606VRDLK, 2720KELRI, 2833LRREK, 2854KRELR, 2914KEKLR, 2916KLREL, 2980QRLLS, 3075KLEKL, 3077EKLLK, 3152QKKYL	Multiple	0	20
65	Vimentin	Human	P08670	466	104KVELQ, 122RFLEQ, 156ELRRQ, 186REKLQ, 270RDVRQ	184RLREK	Multiple	100	20
66	Serine/threonine-protein kinase ULK1	Mice	O70405	1051	227QDLRL, 962FLDKQ, 966QRLLD	62KEIKI, 65KILKE, 67LKELK, 955SLRLQ	Multiple	49.48	38,74
67	E3 ubiquitin-protein ligase TRIM21	Mice	Q62191	470	202QRLEK, 249QEVRI	148EKLRK, 178QKSRI	Multiple	100	38,75
68	Cellular tumor antigen p53	Human	P04637	393	-	200NLRVE, 341FRELN	-	100	4,12,76
69	Epidermal growth factor receptor	Human	P00533	1210	-	79LKTIQ, 208QKLTK, 486QKTKI, 960KFREL, 976QRYLV	-	100	4,77
70	Sorting nexin-10	Human	Q9Y5X0	201	-	100DFLRK	-	100	31,78
71	Citrate synthase, mitochondrial	Mouse	Q9CZU6	464	-	49KTFKQ, 379QLYKI, 464NLKDV	-	100	12,15
72	Myocyte-specific enhancer factor 2A	Human	Q02078	507	-	7QITRI	-	21.1	4,6,79
73	Ubiquitin carboxyl-terminal hydrolase isozyme L1	Human	P09936	223	-	112SVLKQ	-	100	4,6,80,81
74	Peroxisomal multifunctional enzyme type 2	Human	P51659	736	-	80EKVVK, 359KDLKF, 641EVVKK	-	99.73	6,82
75	Translationally-controlled tumor protein	Human	P13693	172	-	19KIREI	-	100	6,83
76	High mobility group protein B1	Human	P09429	215	-	87KKFKD, 150KLKEK	-	100	6,12,83
77	Induced myeloid leukemia cell differentiation protein Mcl-1	Human	Q07820	350	-	234KLDIK	-	59.71	6,84
78	Sterol 26-hydroxylase, mitochondrial	Mouse	Q9DBG1	533	-	285KKLID, 494SRLVQ	-	98.5	6,85
79	Hypoxia-inducible factor 1-alpha	Human	Q16665	826	-	164QRSFF, 373QLFTK, 387FDKLK, 388DKLKK, 389KLKKE, 529NEFKL, 573QLRSF, 768QKTII	-	60.90	12,15
80	Malate dehydrogenase, cytoplasmic	Mouse	P14152	334	-	99KDLLK, 108IFKSQ	-	100	12,13,31,86,87
81	Centrin-1	Human	Q12798	172	-	51KELKV	-	100	12,15
82	ADP-ribosylation factor-like protein 13B	Human	Q3SXY8	428	-	173KKIDK, 221ERVRK	-	53.5	20
83	Histone deacetylase HDAC6	Human	Q9UBN7	1215	-	49EVKKK, 872KKVLE	-	76.63	20
84	DNA damage-inducible transcript 4 protein	Mice	Q9D3F7	229	-	126KELLR	-	100	20
85	Protein c-Fos	Rat	P12841	380	-	186LLKEK	-	16.84	38,88,89
86	Kelch-like ECH-associated protein 1	Human	Q14145	624	-	70LRLSQ, 259QRRFY, 261RFYVQ	-	100	13,90
87	Myocyte-specific enhancer factor 2D	Human	Q14814	521	-	Advanced motif (21FTKRK, 87TLRKK)	-	18.23	36,91
88A	Perilipin-2	Mouse	P43883	425	-	414SLKVQ	-	56	4,6,92,93
88B	Perilipin-2	Human	Q99541	437	-	Advanced motifs (14TRVVN, 236LSRVK, 355SVFRN)	-	54.46	4,12,19,20
89	Protein AF1q	Human	Q13015	90	-	84SFELD (No such motif found using KFERQ finder)	-	0	4,6,18,94
90	Phosphatidylethanolamine-binding protein 1	Human	P30086	187	-	Advanced motifs (153SFRKK)	-	100	4,95
91	Glyceraldehyde-3-phosphate dehydrogenase	Rat	P04797	333	-	Advanced motifs (140NSLKI, 143KIVSN, 320NRVVD)	-	100	13,44,46,96,97
92	Calcipressin-1	Human	P53805	252	-	Advanced motifs (103FKSFK, 104KSFKR)	-	51.98	4,6,13,56,98
93	Glutathione transferase	Species not clear	-	-	-	NKKFE (GST can be a substrate of CMA under specific conditions, such as during ischemia-reperfusion injury where the enzyme, specifically GSTP,S-glutathionylates nitric oxide synthase(eNOS), leading to its degradation via CMA)	-	-	1,71
94	Galectin-3	Human	P17931	250	No such motif found using KFERQ finder	No such motif found using KFERQ finder	-	99.6	4,99
95	Polyubiquitin-C	Human	P0CG48	685	No such motif found using KFERQ finder	No such motif found using KFERQ finder	-	100	20
96	Polyubiquitin-B	Human	P0CG47	229	No such motif found using KFERQ finder	No such motif found using KFERQ finder	-	100	20
97	Ubiquitin-ribosomal protein eL40 fusion protein	Human	P62987	128	No such motif found using KFERQ finder	No such motif found using KFERQ finder	-	100	20
98	Ubiquitin-ribosomal protein eS31 fusion protein	Human	P62979	156	No such motif found using KFERQ finder	No such motif found using KFERQ finder	-	100	20